Banding Techniques

Staining/banding.

What is Banding Technique?

Chromosome banding is an essential technique used in chromosome karyotyping to identify normal and abnormal chromosomes.

What are the types of banding?


a) G-banding .
b) Q-banding
c) C-banding
d) R-banding

Chromosomes have the property of forming alternating dark and light bands.

Fixed metaphase preparation is stained by one of the following banding techniques:
1G-bandingGiemsa bandingMost commonly used method
2Q-bandingQuinacrine bandingUsed to demonstrate
bands along chromosomes.
3C-bandingConstitutive bandingDemonstrate
constitutive heterochromatin.
4R-bandingReverse staining Giemsa banding
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